| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 11 | Rhizobium leguminosarum | TTGCCGATCTGCGCAGAA | AAGGAGAGATCGGCAAGCG | 60.05 | 59.86 | 256 | 78 |
100.00%
|
100.00%
|
| Primer 12 | Rhizobium leguminosarum | TGATGATCGCACACGGCA | CGACGTCTCGTGTTCGGAA | 59.74 | 60.08 | 299 | 74 |
100.00%
|
100.00%
|
| Primer 13 | Rhizobium leguminosarum | TGCGGATGAGATGATCGCC | TTGCGGATCGAGAAGCTGG | 60.01 | 60.15 | 249 | 69 |
100.00%
|
100.00%
|
| Primer 14 | Rhizobium leguminosarum | TGCGGATGAGATGATCGCC | GCGGATCGAGAAGCTGGAA | 60.01 | 59.86 | 247 | 59 |
100.00%
|
100.00%
|
| Primer 15 | Rhizobium leguminosarum | TTTCCTTCGCGTTGCCCT | TCGTCGAGCTCCTGGAAGA | 59.89 | 60.00 | 159 | 53 |
100.00%
|
50.00%
|
| Primer 16 | Rhizobium leguminosarum | TGCTGATGCCCTTTGCCT | TGGTCAGCTTCGACGTGT | 59.56 | 58.87 | 168 | 53 |
100.00%
|
50.00%
|
| Primer 17 | Rhizobium leguminosarum | TGCTGATGCCCTTTGCCT | GCGATGTAGCCGAGGAACA | 59.56 | 59.86 | 271 | 53 |
100.00%
|
50.00%
|
| Primer 18 | Rhizobium leguminosarum | TGCTGATGCCCTTTGCCT | TAGCCGAGGAACAGGGTGT | 59.56 | 60.23 | 265 | 53 |
100.00%
|
50.00%
|
| Primer 19 | Rhizobium leguminosarum | TGCTGATGCCCTTTGCCT | TGTAGCCGAGGAACAGGGT | 59.56 | 60.23 | 267 | 53 |
100.00%
|
50.00%
|
| Primer 20 | Rhizobium leguminosarum | TTCGCGTTGCCCTGGAAA | TCGTCGAGCTCCTGGAAGA | 60.20 | 60.00 | 154 | 48 |
100.00%
|
50.00%
|